FIG 2.

Proinflammatory cytokine production is partly dependent on STING during S. pneumoniae infection in vitro but not in vivo. (A to C) WT and Tmem173^−/− BMDMs were infected with S. pneumoniae for 16 to 18 h, and proinflammatory cytokine production was determined by sandwich ELISA. (D to F) Cytokine protein concentration in the supernatant of cGas^−/− and WT cells infected for 16 to 18 h with S. pneumoniae was measured by sandwich ELISA. (G to I) Cytokine production in whole lung homogenates of S. pneumoniae-infected mice was quantified by ELISA. Data are shown as means ± SD. Data are representative of 4 (A to C) or 3 (D to F) independent experiments carried out in duplicates. (G to I) Data represent analyses of 7 mice. Data were analyzed through the Mann-Whitney U test. Comparisons with a P value of <0.05 were considered significant.