FIG 3.

Endogenous HAQ STING is impaired in mediating type I IFN and proinflammatory cytokine responses to S. pneumoniae infection. PBMCs from healthy volunteers were isolated, cultivated for 7 days, and subsequently infected with S. pneumoniae at a multiplicity of infection of 2.5 or 10 or stimulated with 0.2 or 1 μg/ml of pneumococcal DNA for 6 h. The expression of IFNB (A), IL1B (B), TNFA (C), and IL-6 (D) was assessed by qRT-PCR. Data are represented as means ± SD from 4 independent experiments carried out in triplicates. Comparisons with a P value of <0.05 were considered significant.