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. 2018 Feb 16;5:30. doi: 10.3389/fmed.2018.00030

Figure 3.

Figure 3

Angiogenic metrics from Ov-GRN-1 treatments. ImageJ was used to quantify 12-h time point images for various characteristics of the tubule networks. Treatments consist of the blank control (medium only); anti-angiogenic negative control [10 µM sulforaphane (SFPH)]; pro-angiogenic positive control [1.2 nM vascular endothelial growth factor (VEGF)]; and 5–40 nM Ov-GRN-1 treatments and are plotted relative to the blank (medium only) control treatment. (A) Topological features representing an increase in complexity of the tubule network are measured by the relative number of meshes and (B) branching junctions. (C) Dimensional descriptors of the networked tubules include the number of tubule segments and (D) the total combined length of the tubule segments. All panels: two-way ANOVA with Holm–Sidak’s multiple comparisons test to compare Ov-GRN-1 treatment to medium only blank control: ns = not significant; *P < 0.05; **P < 0.01; ****P < 0.0001. The mean is marked as a horizontal bar with SE bars from 3 to 9 biological replicates plotted as circles. Dotted line crosses the medium only blank 100% value.