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. 2012 May 1;9(2):27–37. doi: 10.1513/pats.201201-008MS

Figure 4.

Figure 4.

Kinetics of rimmed clone formation. Rimmed clones were generated from mice harboring the actin-DsRed transgene or the Ubiquitin-GFP transgene and imaged over time. (A) Culture Day 3. Only single cells are observed (arrow). (B) Culture Day 4. A clone of 2–3 cells (arrow) is detected. C. Culture Day 5. A clone of 16–20 cells is detected. Single cells form a cobblestone epithelium (white arrow) and are the majority of clone cells. A rim (yellow arrow) is forming. (D) Culture Day 7. A rimmed clone with a well developed rim (yellow arrow) is detected. (E) Adjacent rimmed clones, one generated from a DsRed-expressing cell (red) and another from a GFP-expressing cell (green), form a conjoined clone. Note that cells from the two clones do not mix and that a rim domain does not form between the two clones.