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. 2018 Feb 9;14(2):217–227. doi: 10.7150/ijbs.22811

Figure 2.

Figure 2

The combination of TCS and TRAIL promoted apoptosis of TRAIL-resistance cells. (A) Apoptosis was detected by Annexin V-FITC/PI double-staining. Cells were treated with 50 ng/ml TRAIL or/and the IC20 dose of TCS for 48 h. (B) The percentage of apoptotic cells to total cells. Data were presented as column graph of 3 independent experiments with the mean value. *, p < 0.05; **, p < 0.01. (C) After H1299 cells were incubated with 50 ng/ml TRAIL or/and 40 μg/ml TCS for 48 h, the apoptosis of H1299 cells was measured by the TUNEL assay. The magnification was 100X. The scale bar: 200 μm. (D) A graphical representation of the data presented the apoptotic index of 100 cells from 3 independent experiments. *, p < 0.05; **, p < 0.01. (E) The protein levels of apoptosis-related proteins FADD, Caspase 3/8, PARP, BCL-2 and BAX were analyzed by Western blot. GAPDH was used as an internal control to verify equal protein loading.