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. 2018 Jan 1;14(1):1–9. doi: 10.7150/ijbs.23165

Figure 2.

Figure 2

Deletion of β-catenin in HCs resulted in diminished endochondral bone formation. (A) The X-ray images showed an expanded radiolucent area (correlated to the calcified cartilage region, yellow arrow) with an extremely low mineralized ramus in the 2-week old Col10a1-Cre; β-catenin cKO mice. (B) Toluidine blue staining displayed an expansion of all MCC cartilage layers in cKO mice with a particular increase in HCs, but with no trabecular bone present in subchondral bone area. (C) Col 2 IHC combined with cell lineage tracing (Col10a1-Cre began activation at E14.5, in HCs and their transformed bone cells) demonstrated a considerably thickened Col2+ region in cKO mice. (D) Col10+ cells increased in cKO compared with control mice. (E-G) There was only a thin layer of cKO subchondral bone in cKO mice, with a sharp reduction in expression of Runx2 (E), SOST (F) and DMP1 (G) compared with the control mice (arrows).