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. 2018 Jan 11;14(1):57–68. doi: 10.7150/ijbs.22235

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Analysis of viral genome DNA copy numbers in selected tissues After in vivo bioluminescence analysis, animals were killed, genomic DNA was isolated from different tissue samples and 100 ng of each was used in triplicate to determine Firefly luciferase genome copies. The mutant of Y703F showed 3-fold enhancement in liver and kidney tissues and 7-fold higher levels in gastrocnemius (Gas) gene delivery, compared with wild-type AAV8. The data are shown as mean values ± SD, ^*p<0.05 compared with wild-type AAV8groups.