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. 2018 Jan 11;14(1):78–86. doi: 10.7150/ijbs.22426

Figure 3.

Figure 3

Fam60al-AS negatively regulates fam60al expression by forming dsRNA. A: RT-qPCR analysis of fam60al in fam60al-AS overexpressed embryos. Overexpression of fam60al-AS at the sphere stage, resulted in down-regulation of fam60al expression. *P < 0.05; ** P < 0.01; WT: wild type; OE: over expression. B: RNase protection assay analyzing the dsRNA formed in the overlapping region of fam60al and fam60al-AS at the sphere stage and 24 hpf. PCR bands were detected at the sphere stage and 24hpf with RNase A or RNaseOutTM digestion, while no PCR band was observed at the sphere stage and 24hpf following denaturing at 95°C and digestion with RNase A.