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. 2018 Jan 11;14(1):78–86. doi: 10.7150/ijbs.22426

Figure 4.

Figure 4

Establish of fam60al knockout line with TALENs in zebrafish. A: Schematic showing fam60al gene knockout target sites. Target 1 was on exon 2 and Target 2 on exon 4. B: Primer design sites, transcript map and protein coding sequence for fam60al in fam60al-/- line. Primers F1 and R1 were designed to amplify the fam60al gene fragment deleted 2091bp in fam60alw/- and fam60al-/-. Primers F1 and R2 were designed to amplify the wild type fam60al gene fragment in fam60alw/w and fam60alw/-. Fam60al-/- encoded a premature termination 33 amino acids peptide. C: PCR results of different genetype zebrafish. Primer set 1: amplified fragment of primers F1 and R1, Primer set 2: amplified fragment of primers F1 and R2, M: DNA marker, *: fam60al-/-, #: fam60alw/w, and the rest for fam60alw/-.