FIG 3 .

Recombineering with concurrent Cas9-mediated counterselection. (A) Frequency of colonies recovered using different combinations of counterselection vectors, rifampin resistance recombineering oligonucleotide, and selective media when recombineering oligonucleotides and counterselection vectors are introduced concurrently. (B) Fraction of colonies carrying counterselection vectors which display resistance to rifampin after recombineering with cotransformation of counterselection vectors. Error bars in both panels indicate standard errors of the means.