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. 2018 Feb 13;9(1):e00064-18. doi: 10.1128/mBio.00064-18

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Copyright © 2018 Mehta and Laimins.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 1 — Episomal HPV-positive cells lead to DSB formation in a differentiation-dependent manner. (A) Neutral comet assays were performed on differentiated HFK (i) and CIN612 (ii) cells and visualized using a total DNA stain and epifluorescence microscopy. Panels are representative images of comets. (B) Neutral comet assays were performed on undifferentiated (UD) and 72-h calcium-induction-differentiated (D72) HFKs, CIN612 cells, and matched-genetic-background HFK 31 cells, which were visualized with epifluorescence microscopy and quantitated programmatically using the OpenComet software package for FIJI in percent tail DNA. Statistical significance is indicated on the graph and was analyzed using a Student’s t test and results from at least three individual experiments. ****, P < 0.0001; *, P < 0.05. Brackets indicate comparisons between conditions. More than 100 cells were analyzed for each condition across at least three individual experiments.