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. 2018 Feb 13;9(1):e00064-18. doi: 10.1128/mBio.00064-18

FIG 2 .

FIG 2 

HPV oncogenes E6 and E7 induce DSBs by neutral comet assay. (A) Neutral comet assays were performed on undifferentiated or differentiated HFK, HFK 31E6, HFK 31E7, and HFK 31E6E7 cells and visualized using a total DNA stain and epifluorescence microscopy. Panels are representative images of comets. (B) Neutral comet assays were performed on undifferentiated and calcium-induction-differentiated (72-h) HFK, HFK 31E6, HFK 31E7, and HFK 31E6E7 cells, which were visualized with epifluorescence microscopy and quantitated programmatically using the OpenComet software package for FIJI in percent tail DNA. Statistical significance is indicated on the graph and was analyzed using a Student’s t test and results from at least three individual experiments. ****, P < 0.0001; ***, P < 0.001; **, P < 0.01. Statistical significance data indicate increases over the results seen with undifferentiated or differentiated normal keratinocytes. Brackets indicate statistical comparisons between E6 and E6/E7. More than 100 comets were analyzed for each condition across at least three individual experiments.