Fig 2. Translation of incoming genomes is attenuated in IFN pre-treated cells.

(A) Schematic of translation reporter RNA with wild-type virus 5’ and 3’ NTRs. (B). MEF cells were untreated or pre-treated with 0, 10, 100 or 1000 IU mouse IFN for 16h and electroporated with reporter RNAs of indicated viruses as described in Materials and Methods. Cells were lysed 2h post-electroporation and luciferase activity was measured. Data is RLUs per μg total protein expressed as a fold change over no IFN; average of three experiments for per treatment. ***, P<0.0015; ****, P<0.0001 using One way ANOVA. All error bars are standard deviations.