Fig 4. Host macromolecular synthesis shutoff by VEEV mutants.
(A-D) Neuro 2a cells were infected with indicated viruses (M.O.I. = 2.5) and labeled with 100μCi/ml of [35S] Cys/Met for 2h at 6h (A) and 18h (B) p.i. Lysates were collected and resolved on SDS-PAGE gels and visualized as described in Materials and Methods. (C and D) Densitometry performed on gels from (A) and (B) respectively. ****, P<0.0001; **, P<0.01; *, P<0.05 using One-way ANOVA, compared to VEEV WT. Ns = not significant. Data is representative of two independent experiments. All error bars are standard deviations.