Table 1.

ISHH probe design.

Target mRNA	Riboprobe length	PCR primers	Nucleotide target	GenBank mRNA accession #
PPE	817 bp	Sense: 5′-TTCCTGAGGCTTTGCACC-3′	312–1128	001002927
		Antisense: 5′-TCACTGCTGGAAAAGGGC-3′
PPT	900 bp	Sense: 5′-TCGAACATGAAAATCCTCGTGGCC-3′	95–994	D17584
		Antisense: 5′-CACATCATACAATGACTGAAGACC-3′
D1	694 bp	Sense: 5-CTCATAAGCTTTTACATCCCCG-3	1116–1809	NM_010076
		Antisense: 5-GAGACATCGGTGTCATAGTCCA-3
D2	1086 bp	Sense: 5′-GAAGATCCTGCACTGCTGAGT-3	1412–2497	NM_010077.1
		Antisense: 5′-ATGTTACAGAGTTGGAGCCCAG-3′
BDNF	920 bp	Sense: 5′-GGCGCCCATGAAAGAAGTAAAC-3′	715–1634	MN_007540
		Antisense: 5′-CGGCAACAAACCACAACATTAT-3′