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. Author manuscript; available in PMC: 2018 Feb 21.
Published in final edited form as: Methods Mol Biol. 2016;1389:111–126. doi: 10.1007/978-1-4939-3302-0_7

Figure 2. Setting classifiers for compensation controls.

Figure 2

(A) Compensation classifiers for the SpectrumGreen probe that is detected in channel 2. All channels are enabled (yellow box) and an Intensity Lower Limit of channel 2 is selected to collect events with a positive signal (red box). (B) Compensation classifiers for the DAPI nuclear dye that is detected in channel 7. All channels are again enabled and an Intensity Lower Limit of channel 7 is selected to collect events with a positive signal (red box).