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. 2018 Feb 8;3(3):e95396. doi: 10.1172/jci.insight.95396

Figure 5. Swiprosin-1 deficiency attenuates the activation of the JAK2/STAT1/STAT3 pathway and the expression of IFN-γR in LPS-treated peritoneal macrophages.

Figure 5

(A) The activation of the JAK/STAT pathway in macrophages after LPS treatment (n = 3, *P < 0.05,**P < 0.01 WT-LPS vs. WT-0 hour; ┼P < 0.05, WT-LPS vs. KO-LPS at the same time point), 1-way ANOVA (LSD test); the results are depicted as the mean ± SEM. (B) JAK/STAT signaling activation by LPS in swiprosin-1 overexpressed RAW264.7 cell (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, GFP-LPS vs. GFP-0 hour; ##P < 0.01, ###P < 0.001, OE-LPS vs. OE-0h; ┼┼P < 0.01, ┼┼┼P < 0.001, GFP-LPS vs. OE-LPS at the same time point), 1-way ANOVA (LSD test); the results are depicted as the mean ± SEM. (C) Serum level of IFN-γ after LPS treatment for 6 hours (n = 5–9, ***P < 0.001, WT-LPS vs. WT-CON; ┼┼┼P < 0.001, WT-LPS vs. KO-LPS), 1-way ANOVA (LSD test); the results are depicted as the mean ± SEM. (D) IL-1β, IL-6, and TNF-α levels in the macrophages after IFN-γ treatment for 12 hours (n = 3, **P < 0.01, ***P < 0.001 WT-treatment vs. WT-CON; ###P < 0.001, KO-treatment vs. KO-CON; ┼┼P < 0.01, ┼┼┼P < 0.001, WT vs. KO with same treatment), 1-way ANOVA (LSD test); the results are depicted as the mean ± SEM.