Skip to main content
. 2018 Feb 8;3(3):e95417. doi: 10.1172/jci.insight.95417

Figure 3. CX3CL1 rescues CX3CR1-WT/WT and CX3CR1-WT/M280, but not CX3CR1-M280/M280, CD14+ monocytes from serum starvation–induced death.

Figure 3

(A) Representative FACS histograms of propidium iodine (PI) staining in CX3CR1-WT/WT (upper panels) and CX3CR1-M280/M280 (lower panels) CD14+ monocytes following serum starvation in the presence or absence of 100 nM of CX3CL1. (B) Percent of dead CD14+ monocytes elicited by serum starvation in the presence or absence of 100 nM of CX3CL1 in CX3CR1-WT/WT (left panel), CX3CR1-WT/M280 (middle panel), and CX3CR1-M280/M280 (right panel) cells. Shown are paired experimental results with or without CX3CL1. (C) The percent decrease in cell death conferred by CX3CL1 exposure in serum-starved CD14+ monocytes is greater in CX3CR1-WT/WT compared with CX3CR1-WT/M280 cells, while no CX3CL1-induced decrease in cell death is seen in CX3CR1-M280/M280 cells. n = 22 CX3CR1-WT/WT, 11 CX3CR1-WT/M280 and 6 CX3CR1-M280/M280. *P < 0.05; ***P < 0.001; ****P < 0.0001. Statistical analysis was performed using paired 2-tailed t tests (B) or 1-way ANOVA with Tukey’s multiple comparisons test (C). Quantitative data represent the mean ± SEM.