Figure 1. Downregulation of human β cell identity in PolyI:C-transfected EndoC-βH1 cells.

EndoC-βH1 cells were either mock transfected (control) or transfected with PolyI:C and analyzed 24 hours later. (A and B) Heatmap from global transcriptomic analysis and RT-qPCR data represent downregulated β cell genes (n = 3). (C and D) Western blot analysis (representative of 3 independent experiments) and quantification of MAFA expression (n = 3). (E) EndoC-βH1 cells were either mock transfected (control, white) or transfected with PolyI:C (gray) and glucose-stimulated insulin secretion (GSIS) was performed 6 days later with 0 or 20 mM glucose. Insulin secreted in ng/10⁶ cells is represented (n = 3). Data from RT-qPCR, Western blot, and GSIS analyses represent the mean ± SD of 3 independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 relative to BSA by 1-way ANOVA with Bonferroni’s correction.