Figure 2. IKKβ regulates adipocyte and osteoblast differentiation of MEFs.

(A–E) MEFs isolated from IKKβ^F/F mice were infected with control lentivirus or lentivirus expressing Cre. (A) Immunoblotting for IKKβ proteins in MEFs. (B and C) Oil-red-O staining (B) and qPCR analysis (C) of MEFs induced by an adipogenic cocktail (n = 3). (D and E) ALP staining (D) and qPCR analysis (E) of MEFs induced by an osteogenic cocktail (n = 3). Scale bar: 100 μm. Error bars represent ± SEM. Significance was determined by Student’s t test (C and E). *P < 0.05; **P < 0.01.