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. 2018 Jan 25;3(2):e96660. doi: 10.1172/jci.insight.96660

Figure 5. Deficiency of β-catenin abolishes the impact of IKKβ expression on adipogenesis and osteogenesis of MSCs.

Figure 5

(A) Immunoblotting for β-catenin and IKKβ proteins in control or β-catenin–deficient C3H/10T1/2 cells infected with control or WT IKKβ virus. (B and C) Oil Red O staining (B) and qPCR analysis (C) of control and β-catenin–deficient C3H/10T1/2 cells induced by an adipogenic cocktails (n = 3). Scale bar: 100 μm. (D and E) ALP staining (D) and qPCR analysis (E) of control and β-catenin–deficient C3H/10T1/2 cells induced by an osteogenic cocktails (n = 3). Scale bar: 100 μm. Error bars represent ± SEM. Significance was determined by 2-way ANOVA (C and E). **P < 0.01, ***P < 0.001.