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. 2018 Jan 25;3(2):e96660. doi: 10.1172/jci.insight.96660

Figure 8. IKKβ phosphorylates β-catenin to prime it for β-TrCP-mediated ubiquitination.

Figure 8

(A) GST–β-catenin and indicated mutant proteins were phosphorylated by IKKβ in vitro and analyzed by immunoblotting using anti–phospho-ser33, -ser37 or -ser45 β-catenin antibodies. (B) Immunoblotting for phosphorylated β-catenin proteins in C3H/10T1/2 cells infected with control, WT IKKβ, and IKKβ KM virus. (C) Immunoblotting for phosphorylated β-catenin proteins in C3H/10T1/2 cells treated with vehicle or FFAs. (D) Immunoblotting for phosphorylated β-catenin proteins in control or IKKβ-deficient C3H/10T1/2 cells treated with vehicle or LPS. (E) GST–β-catenin and indicated mutant proteins were phosphorylated by IKKβ in vitro. The reaction substrates were subjected for cell-free ubiquitination assays and analyzed by immunoblotting.