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. 2018 Jan 25;3(2):e96660. doi: 10.1172/jci.insight.96660

Figure 9. Prrx1Cre-mediated IKKβ deficiency decreases adipogenesis and increases osteogenesis in BMMSCs.

Figure 9

(A) Immunoblotting for IKKβ proteins in BMMSCs of IKKβF/F and Prrx1Cre+IKKβF/F mice. (B–G) BMMSCs were isolated from IKKF/F and Prrx1Cre+IKKβF/F mice. Oil Red O staining (B) and qPCR analysis (C) of BMMSCs induced by an adipogenic cocktail (n = 3). ALP staining (D) and qPCR analysis (E) of BMMSCs induced by an osteogenic cocktail (n = 3). Immunoblotting for ubiquitinated β-catenin (F) and nuclear β-catenin proteins (G) of isolated BMMSCs. Scale bar: 100 μm. Error bars represent ± SEM. Significance was determined by Student’s t test (C and E). *P < 0.05; **P < 0.01.