Figure 1. Immune responses in iRhom2-mutant adult mouse hearts after myocardial infarction.

Bar graphs of immune cell populations distinguished by flow cytometry staining. (A) Reparative (CD45⁺CD64⁺MerTK^–Ly6G^–Ly6C^hi) and (B) proinflammatory (CD45⁺CD64⁺MerTK^–Ly6G^–Ly6C^hi) monocyte numbers were not significantly changed in iRhom2-deficient (iRhom2^–/–) hearts compared with wild-type controls following injury. (C) Persistence of neutrophils (CD45⁺CD64^–MerTK^–Ly6G⁺) was observed in iRhom2 mutants, which is significant by 7 days after injury. (D) iRhom2^–/– mice exhibited an increase in the overall number of macrophages (CD45⁺CD64⁺MerTK⁺Ly6G^–F4/80⁺) at day 2, 4, and 7 after myocardial infarction compared with wild-type mice. Data are shown as the mean ± SEM, n = 5 per experimental group. *P ≤ 0.05, **P ≤ 0.01, 1-way ANOVA and post-hoc test.