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. 2018 Feb 8;3(3):e96219. doi: 10.1172/jci.insight.96219

Figure 1. Continuous IL-15 treatment results in increased proliferation and decreased survival.

Figure 1

(A) Schematic of the experimental system used to study effect of continuous IL-15 treatment (IL-15cont) versus intermittent IL-15 treatment (IL-15gap) on NK cells. Black bars represent IL-15 treatment, white bars represent incubation with media alone. (B) Upon harvest, the number of viable NK cells/ml of culture was assessed (n = 16). (C) NK cells were labeled with CellTrace Dye (Molecular Probes), and proliferation was assessed at day 9. Representative histogram showing CellTrace dilution in CD56+CD3– NK cells in the IL-15cont group (light gray) and the IL-15gap group (dark gray). (D) The proliferation index was calculated for NK cells based on the CellTrace dilution data utilizing Flowjo software (n = 4). (E) NK cells were assessed for cell death by gating to assess the percentage of cells incorporating Live/Dead Fixable dye (n = 12). (F) Fas and (G) FasL MFI was assessed on NK cells (n = 12). Paired t test was used to compare samples. *P ≤ 0.05; ***P < 0.001.