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. 2018 Jan 11;3(1):e95882. doi: 10.1172/jci.insight.95882

Figure 3. AID deficiency promotes the lymphoid expansion and proliferation in spleen and lymph nodes.

Figure 3

(A) Enlargement of lymphoid organs and expansion of lymphocytes. Images of representative spleens, PLN, and MLN of 8-week-old female AID–/–NOD and AID+/+NOD mice and the summary of total lymphocyte count are shown. Scale bar: 6 mm. (B) Total numbers of B220+CD19+ B cells, CD4+ T cells, and CD8+ T cells in spleens of 8-week-old female AID–/–NOD and AID+/+NOD mice analyzed by flow cytometry. (A and B) Data were pooled from 3 independent experiments and are shown as mean ± SEM. n ≥ 9 mice/group. (C) BrdU incorporation was performed in spleen and PLN cells in 8-week-old female AID–/–NOD and AID+/+NOD mice. The representative flow cytometric plots and the summarized percentage of BrdU+ lymphocytes are shown. Individual data and mean ± SEM of 1 of the 2 independent experiments are shown. n = 4 mice/group. *P < 0.05; **P < 0.01; ***P < 0.001, multiple t test. PLN, pancreatic lymph nodes; MLN, mesenteric lymph nodes; PP, Peyer’s patches; SSC, side scatter.