(A and B) b-Waves and a-waves in animals with pan-retinal deletion of FAS at P28 and P60 compared with controls under light-adapted and dark-adapted conditions (n = 8–10 animals/group, 2-way ANOVA). (C and D) Representative ERG tracings in scotopic (C) and photopic (D) conditions displaying marked reductions in b-wave and a-wave amplitudes across multiple stimulus luminances. (E and F) b-Waves and a-waves in animals with loss of FAS in rod photoreceptors alone (n = 6 animals/group, 2-way ANOVA). (G and H) Representative ERG tracings in scotopic (G) and photopic (H) conditions for mice with loss of FAS in rod photoreceptors alone. (I) b-Waves and a-waves in eyes with FAS deficiency in endothelial cells (n = 15 animals/group, 2-way ANOVA). (J) Effects of dark-rearing (left panel) or dietary excess of palmitate (right panel) on ERG tracings in pan-retinal FASKO mice at P60 (n = 8–10 animals/group; 2-way ANOVA). **P < 0.01, ***P < 0.001, ****P < 0.0001.