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. 2018 Feb 8;3(3):e93203. doi: 10.1172/jci.insight.93203

Figure 2. Analysis of cultured cells from patients with COPD and controls.

Figure 2

(A) Percentage of β-Gal–positive cells and protein levels of Akt phosphorylated at Ser473 (Akt-Ser473), glycogen synthase kinase 3 (GSK3), S6 kinase (S6K), and 4E-binding protein 1 (4E-BP1) proteins in cultured pulmonary artery smooth muscle cells (PA-SMCs) from 11 controls and 12 patients with COPD at the earliest cell passage. Values are mean ± SEM. **P < 0.01 vs. controls, by 2-tailed t test. Representative photographs of PA-SMCs from patients with COPD and controls stained for senescence-associated β-Gal activity at the corresponding cell passage (left panel; original magnification, 10×). Representative photographs of PA-SMCs from patients with COPD and controls costained for p-Akt and p16 (right panels), illustrated with their respective gels. Scale bars: middle panel 200μm and right panel 25 μm. (B) Similar representations for pulmonary vascular endothelial cells (P-ECs) from 12 patients with COPD and 12 controls. These measurements were performed in cells deprived of serum for 24 hours (P-ECs) or for 48 hours (PA-SMCs).