REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Monoclonal ANTI-FLAG M2 antibody	Sigma	F9291-1MG
Anti-GAPDH antibody	Abcam	ab9485
Amersham ECL Mouse IgG, HRP-linked Ab (from sheep)	GE Healthcare Life Sciences	NXA931-1ml
Bacterial and Virus Strains
One Shot TOP10 chemically competent E. coli	Life Technologies	C404006
Biological Samples
DMEM	VWR	45000-304
Opti-MEM	Life Technologies	31985062
FBS	Clontech	631367
Chemicals, Peptides, and Recombinant Proteins
[γ-32P]ATP	PerkinElmer	NEG035C001MC
[α-32P]UTP	PerkinElmer	NEG007H001MC
[α-32P]GTP	PerkinElmer	NEG006H500UC
Pronase	EMD Millipore	537088-50KU
Notl-HF	New England Biolabs	R3189L
cOmplete, Mini, EDTA-free protease inhibitor cocktail tablets	Sigma	11836170001
TRI Reagent solution	Life Technologies	AM9738
Phenol:chloroform:isoamyl alcohol 25:24:1	Sigma	P2069-100ML
Chloroform	J.T.Baker Analyical	9180-01
SUPERase-In	Life Technologies	AM2696
TURBO DNase	Life Technologies	AM2239
Yeast tRNA	Life Technologies	15401011
Dynabeads MyOne Streptavidin C1	Life Technologies	65002
ANTI-FLAG M2 magnetic beads	Sigma	M8823
3X-FLAG peptide	Sigma	F4799-4MG
GE Healthcare Amersham Hybond-XL membranes	Thermo Fisher Scientific	45001147
GE Healthcare Amersham Hybond-NX membrane	VWR	95038-412
Whatman Protran nitrocellulose membrane	Sigma	Z670898
PVDF pre-cut blotting membrane, 0.2 μm pore size	Life Technologies	LC2002
GE Healthcare Amersham Hyperfilm ECL	Thermo Fisher Scientific	45001504
GE Healthcare Amersham ECL prime western blotting detection reagent	Thermo Fisher Scientific	45002401
Gel loading buffer II (denaturing PAGE)	Life Technologies	AM8547
Whatman Nytran SuPerCharge (SPC) TurboBlotter	Sigma	WHA10416302
Critical Commercial Assays
mMESSAGE mMACHINE SP6	Life Technologies	AM1340
RNeasy Mini Kit	QIAGEN	74104
QuikChange Lightning Multi Site-Directed Mutagenesis	Agilent	210515
Phusion High-Fidelity DNA polymerase	New England Biolabs	M0530L
T4 PNK	New England Biolabs	101228-172
MAXIscript T7 RNA polymerase	Life Technologies	AM1312
Vaccinia capping system	New England Biolabs	M2080S
Lipofectamine 2000	Life Technologies	11668019
NuPAGE Novex 4-12% Bis-Tris Gel 1.0 mm	Life Technologies	NP0321BOX
NuPAGE MOPS SDS running buffer	Life Technologies	NP0001
NuPAGE transfer buffer	Life Technologies	NP00061
Micro Bio-Spin P-30 gel columns	Bio-Rad	7326250
NorthernMax-Gly kit	Life Technologies	AM1946
ULTRAhyb Ultrasensitive hybridization buffer	Life Technologies	AM8670
ULTRAhyb-Oligo hybridization buffer	Life Technologies	AM8663
Deposited Data
Raw data	This paper, Mendeley Data	https://data.mendeley.com/datasets/fjg3b788k5/draft?a=f466501d-4dac-4b86-8d57-4cecb1fc3376
Experimental Models: Cell Lines
Human: HEK293T	ATCC	CRL-3216
Experimental Models: Organisms/Strains
Zebrafish	ZIRC	AB
Oligonucleotides
See Table S1	This study	Table S1
Recombinant DNA
pCS2+-eGFP	This study	N/A
pCS2+-drAgo2	This study	N/A
pCS2+-FLAG3-drAgo2	This study	N/A
pCS2+-FLAG3-drAgo2D–A	This study	N/A
pCS2+-FLAG3-drAgo2D–E	This study	N/A
pCS2+-FLAG3-drAgo2Y–F	This study	N/A
pCS2+-FLAG3-drAgo2DY–EF	This study	N/A
pCS2+-FLAG3-hsN-drAgo2	This study	N/A
pCS2+-hsAgo2	This study	N/A
pCS2+-FLAG3-hsAgo2	This study	N/A
pCS2+-FLAG3-hsAgo2D–A	This study	N/A
pCS2+-FLAG3-hsAgo2E–D	This study	N/A
pCS2+-FLAG3-hsAgo2F–Y	This study	N/A
pCS2+-FLAG3-hsAgo2EF–DY	This study	N/A
pCS2+-FLAG3-drN-hsAgo2	This study	N/A
pCS2+-Zeocin	This study	N/A
pCS2+-Zeocin-miR-430	This study	N/A
pCS2+-Zeocin-miR-430-10–11 mm	This study	N/A
pCS2+-Zeocin-miR-430-G–G mm	This study	N/A
pMAX GFP	Amaxa	VDF-1012
pcDNA3-drAgo2	This study	N/A
pcDNA3-FLAG3-drAgo2	This study	N/A
pcDNA3-FLAG3-drAgo2D–E	This study	N/A
pcDNA3-FLAG3-drAgo2Y–F	This study	N/A
pcDNA3-FLAG3-drAgo2DY–EF	This study	N/A
pcDNA3-FLAG3-drAgo2D–A	This study	N/A
pcDNA3-hsAgo2	This study	N/A
pcDNA3-FLAG3-hsAgo2	This study	N/A
GFP-3xPTmiR-1	Giraldez lab	N/A
Software and Algorithms
MATLAB	Mathworks
Multi Gauge 2.2	Fujifilm
GraphPad Prism	GraphPad Software
Other