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. 2018 Feb 21;13(2):e0190893. doi: 10.1371/journal.pone.0190893

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© 2018 Tchernookova et al

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Fig 3 — (A) 1 μM ATP increased intracellular calcium as reported by changes in the fluorescence of the calcium indicator Oregon Green. Subsequent ATP-induced alterations in Oregon Green fluorescence were blocked by 1 μM thapsigargin applied for 15 minutes. (B) A representative single self-referencing recording from an isolated Müller cell demonstrating that thapsigargin also reduced the increase in H+ flux induced by 1 μM ATP from Müller cells; asterisk: background control. (C) Mean responses from imaging data for ATP induced responses in 2-APB, U73122 and thapsigargin. Data was normalized to the last fluorescence data point before treatment. (D) Mean responses with self-referencing. ATP readings represent absolute recorded values minus values of the standing flux.