Fig 5. The cnr1 transcript is enriched in the dorsolateral striatum and is expressed in both striosomes and matrix.

(A) In situ hybridization with a probe for cnr1 in a CalDAG-GEFI-eGFP mouse (with matrix-enriched eGFP) shows striatal enrichment of cnr1 in this coronal hemisection. (B) Magnification of (A). (C) In a section nearby to (A) in this matrix-eGFP mouse, in situ hybridization with anti-sense gfp probes was used to define striosomes (outlined in magenta), based on low probe density. Comparison of the striosome and matrix regions defined in (C) to the widespread distribution of cnr1 label in (B) indicates that cnr1 is expressed in both striosomes and matrix. Note the subcallosal streak, a striosome-rich region that lies just under the corpus callosum. (D) In a section that was labeled for both cnr1 (blue) and gfp (red), single-labeled cnr1-positive cells (examples at arrows) are visible within the striosome-enriched subcallosal streak, along the border with the corpus callosum (designated by a thin line), and double-labeled cells (examples at arrowheads) are visible in the matrix.