Fig 4. Sepsis results in a sustained expansion of MDSCs.

Mice were injected with PBS i.p. (Sham) or subjected to sepsis induction. IL-7 (Sepsis + IL-7) or PBS (Sepsis + PBS) was injected daily for 5 days from day 5–9 post sepsis induction. Gr1⁺CD11b⁺ cells from the spleen and bone marrow were analysed 1 week, 1 month and 3.5 months after sepsis induction. (A, B) Representative flow cytometry images from spleen (A) and bone marrow (B) from analysis after 3.5 months. (C) Frequency of Gr1⁺CD11b⁺ cells among total spleen cells (top) and among total bone marrow cells (bottom). (D) Number of Gr1⁺CD11b⁺ cells in spleen (top) and bone marrow (bottom). n = 6–9 (1 week), 5–12 (1 month), 8–20 (3.5 months). *P< 0.05, **P< 0.01, ***P< 0.001 (ANOVA). Data are expressed as mean ± SEM. Data are representative of three experiments.