Fig 7. Intracellular production of ROS during disease development in pathosystems I-III.

ROS production as visualized using H₂DCF-DA in control and infected potato sprouts, soybean hypocotyls and leaves along with mycelia of control R. solani AG-3, AG-4, AG1-IA, grown on half-strength PDA overlaid with cellophane membranes. (A-B) Control mycelia with no evidence of endogenous ROS at 120 h, (G-H) 36 h, and (M-N) 18 h upon growth on PDA membrane overlaid with cellophane membranes. (C-D) Control S. tuberosum sprout, (I-J) G. max hypocotyl and (O-P) G. max leaf with no evidence of endogenous ROS. Mycelia of R. solani during disease development in pathosystems I-III (Fig E-F, Fig K-L, and Fig Q-R, respectively). Bar = 500 μm.