. 2018 Feb 21;13(2):e0192682. doi: 10.1371/journal.pone.0192682

Table 1. List of primers used in this study.

Target organism	Primer	Sequence (5′ → 3′)	Annealing temperature (°C)	Amplicon size (bp)	Reference
	PDX1 (Pyridoxine biosynthesis gene)
Soybean	GM^a- PDX1.1-F	`TACAGTGTACGGCAACGGTGCA`	59	235	XM_003542937.3
	GM-PDX1.1-R	`GTCCTTGATGAGCTGCGGG`	59	235
	GM-PDX1.2-F	`TACAGTCTACGGCAACGGCGCC`	54	235	NM_001251112.2
	GM-PDX1.2-R	`ATCCTTGATGAGCTGCGGA`	54	235
Potato	ST^b-PDX1.1-qF	`CTGTGACTATTCCTGTAAT`	55	83	[37]
	ST- PDX1.1-qR	`GTAATCTACTCCGATAGC`	55	83
	ST-PDX1.2-qF	`TGCTCTAATCCTTACAAG`	55	162
	ST-PDX1.2-qR	`GTAGGTCTCATCACTAAC`	55	162
*R. solani* AG3**	5'GSP1-(PDX1)	`TGACGAACAGCCTCGACAACATTTCC`	55	219	[27]
	3'GSP2-(PDX1)	`TCCGTTTGTCTGTGGGGCTACATCTCTC`	55	219
*R. solani* AG4**	Rsol^cAG8-PDX1- F	`ATGATCAAAGAGATCGTGGAC`	53	243	AVOZ01001318.1
	RsolAG8-PDX1-R	`GATCATTGCTGCACCTTCGGA`	53	243
*R. solani* AG1-IA**	RsolAG1-IA-PDX1-F	`ATCTCTGCCTACTACCAATG`	51	131	AFRT01001225.1
	RsolAG1-IA-PDX1-R	`TGCCCCCATCCGTGTTCTGAG`	51	131
	PDX2 (Pyridoxine biosynthesis gene)
Soybean	GM-PDX2-F	`TTAGGAGTGAAAGGTGTGGAG`	53	328	XM_014774652.1
	GM-PDX2-R	`TTTGGAGACGAGCTCTGGCAC`	53	328	XM_014774652.1
Potato	ST-PDX2-qF	`ATTCCAATCCTGCTATTC`	55	185	[37]
	ST-PDX2-qR	`CACAATATCAGAAGTTCCT`	55	185
R. solani AG3	3'GSP1-(PDX2)	`AATCTTGCTTGCCTCTGGTGGTGTTG`	55	225	[27]
	5'GSP1-(PDX2)	`ATCCCATTAAATGGTCGGTCCTCATCA`	55	225
R. solani AG4& R. solani AG1-IA	RsolAG8-PDX2-F	`ATTATATCACGCGTGACACC`	53	207	JATN01000314.1
	RsolAG8-PDX2-R	`GGCGCCTTCAACACCACCAGA`	53	207
	PLR (PYRIDOXAL REDUCTASE)
Soybean	GM-PLR-F	`TCCTCCAAAGCCTGAACCCGA`	53	115	XM_003527250.3
	GM-PLR-R	`TAGAAGGAGTTCGTTGGTGT`	53	115
Potato	ST-PLR-F	`GCCGCCCAAACCCGAACCCGA`	58	118	XM_006358747.2
	ST-PLR-R	`AAGGAGGATCTCGTTAGTGT`	58	118	XM_006358747.2
*R. solani* AG3**	PLR AKR8 F	`GAAAGCCTCCTCTTGGAATCT`	58	300	[25]
	PLR AKR8 R	`GGGTAAGATTGGATCGATTGGG`	58	300
*R. solani* AG4 & R. solani AG1-IA**	RsolAG1-IA-PLR-F	`AAGGGGCACTAACCGGAAAGC`	58	194	AFRT01000983.1
	RsolAG1-IA-PLR-R	`ATGTTGCCCGAGAGAGGAGAC`	58	194
	CATALASE
Soybean	GM-CAT ^d-F	`ACATGTTCACCTTTTTATTTG`	47	149	NM_001253092
	GM-CAT-R	`CTTTATACCACTAGTGGTCTT`	47	149
Potato	ST-CAT-F	`ATGTTCACTTTCCTCTTCGAC`	52	147	AY442179.2
	ST-CAT-R	`CTTGACACCACATGTGGGCTT`	52	147
*R. solani* AG3**	RsolAG3-CAT-F	`ACCAGAAGTGTTAGTCCAGCGG`	56	190	[27]
	RsolAG3-CAT-R	`CATCCGGTCACAGCAGCGTA`	56	190
R. solani AG4& *R. solani* AG1-IA**	RsolAG1-IA-CAT-F	`TGCGGGACTCATGCTGCTCTC`	55–54	114	AFRT01002805.1
	RsolAG1-IA-CAT-R	`TCCTTGGTGCCTTTCCTATCC`	55–54	114	AFRT01002805.1
	GLUTATHIONE S-TRANSFERASES
Soybean	GM-GST ^e-F	`TCCATTTGGGATGAGGGTCAG`	53	208	NM_001251762
	GM-GST-R	`ATTTCTGTCATTCCAAACCTCC`	53	208
Potato	ST-GST-F	`TCCTTTTAGCCATAGAGTTGA`	51	200	POTPR1A
	ST-GST-R	`CTTCAAATGCCTCATCAATGC`	51	200
*R. solani* AG3**	RsolAG3-GST-F	`AGAAGACGAGGCAAATGCGA`	57	256	[27]
	RsolAG3-GST-R	`ATCTCTTCAACCGCCTTCCAGT`	57	256
*R. solani* AG4 & R. solani AG1-IA**	RsolAG1-IA-GST-F	`TACAAACGCATACCTTATCGC`	53	217	ELU40094.1
	RsolAG1-IA-GST -R	`TTGGAGCCGGGTAAGTTTTG`	53	217	ELU40094.1
	Reference genes
Soybean	UKN2^f _F	`GCCTCTGGATACCTGCTCAAG`	58	79	[38]
	UKN2_R	`ACCTCCTCCTCAAACTCCTCTG`	58	79
Potato	ST-Actin7-qF	`GGCTATGTATGTTGCTAT`	55	186	[37]
	ST-Actin7-qR	`ATCTTCATCAGGTTATCAG`	55	186
*R. solani* AG3**	G3PDH^g -F	`GGTATTATTGGATACACTGA`	55	129	[25]
	G3PDH -R	`TTAAGCCTCAGCGTCTTTCT`	55	129
*R. solani* AG1-IA**	RsolH3^h _F	`CTTCCAATCATCGGCAGTCCTC`	52	76	[34]
	RsolH3 _R	`ATTGGTATCTTCGAACAAAGACACGAG`	52	76
*R. solani* AG4**	GM-RS-4ⁱ	`CGGTTCATCTGCATTTACCTT`	55	88	[39]
	GM-RS3-R	`AGTGTTATGCTTGGTTCCACT`	55	88
	Transformation
*R. solani* AG3**	RsolAG3-PDX1-CDS-F RsolAG3-PDX1-CDS-R	`GCGGCCGCATCATGTCTGCTCCTGTGTC` `GCGGCCGCCACTCACATAGTCAGCCCAA`	55	969	KF620111.1
*R. solani* AG3**	RsolAG3-PDX2-CDS-F	`GCGGCCGCTCGATGACTAGAACTGAAAC`	55	747	KF620112.1
	RsolAG3-PDX2-CDS-R	`GCGGCCGCGGTTCACTTGGCAAAGCTC`

a Glycine max

b Solanum tuberosum

c Rhizoctonia solani

d Catalase

e Glutathione S-Transferase

f Hypothetical protein unknown 2

g G3PDH: Glyceraldehyde-3-phosphatedehydrogenasegen

h Histone 3

i ITS1 of 5.8S rRNA