Table 4. QTLs detected for plant height-related traits in the two RIL populations across two environments and two ethylene treatments.
| Traits | QTL | Chr. | Location a | Treatment b | Position c (cM) | Marker Interval d | Support interval e (cM) | Physical interval f (bp) | LOD g | R2 (%) h | Ai | Positive allele j |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| KUI3 × B77 | ||||||||||||
| PH | q2PH2-1 | 2 | LS | CK | 49.2 | PZE-102038161- SYN29038 | 47.1–51.7 | 18,316,951–20,724,795 | 3.47 | 6.45 | -3.48 | B77 |
| LS | ET | 8.23 | 14.91 | -5.06 | ||||||||
| WQ | CK | 4.26 | 7.96 | -4.23 | ||||||||
| WQ | ET | 4.01 | 7.38 | -3.99 | ||||||||
| q2PH2-2 | 2 | WQ | CK | 68.7 | PZE-102056514- ZM012191-1494 | 66.9–69.1 | 34,539,814–37,198,403 | 5.92 | 11.31 | -6.43 | B77 | |
| LS | CK | 3.68 | 6.97 | -3.69 | ||||||||
| q2PH2-3 | 2 | WQ | CK | 118.9 | PZE-102132131- PZE-102134800 | 119.2–126.3 | 182,620,244–184,968,231 | 4.23 | 8.12 | 5.41 | KUI3 | |
| WQ | ET | 3.62 | 6.97 | 3.85 | ||||||||
| LS | CK | 5.24 | 9.25 | 5.63 | ||||||||
| LS | ET | 3.26 | 5.84 | 2.97 | ||||||||
| q2PH2-4 | 2 | WQ | CK | 157.2 | PZE-102169752- PZE-102170996 | 156.6–157.8 | 213,205,091–214,210,573 | 2.59 | 4.8 | -4.05 | B77 | |
| q2PH2-5 | 2 | LS | CK | 180.9 | PZE-102184387- SYN15855 | 178.5–180.9 | 227,531,180–228,895,610 | 2.63 | 4.81 | -3 | B77 | |
| LS | ET | 2.89 | 5.12 | -2.68 | ||||||||
| WQ | CK | 3.12 | 5.01 | -4.16 | ||||||||
| WQ | ET | 2.67 | 6.23 | -3.37 | ||||||||
| q2PH3 | 3 | LS | ET | 118.2 | SYN37387- PZE-103144159 | 116.5–119.8 | 195,935,474–199,484,261 | 3.9 | 6.74 | 3.41 | KUI3 | |
| q2PH5 | 5 | WQ | CK | 164.4 | PZE-105156919- PZE-105158393 | 162.3–165.8 | 205,567,299–206,188,028 | 3.66 | 6.93 | 4.77 | KUI3 | |
| LS | CK | 4.02 | 7.71 | 3.95 | ||||||||
| q2PH6 | 6 | WQ | CK | 97.2 | PZE-106094294- PZE-106098403 | 95.2–101.2 | 149,598,681–152,167,281 | 5.37 | 10.31 | -5.67 | B77 | |
| q2PH7 | 7 | LS | ET | 78.5 | PZE-107058976- PZE-107060141 | 78.2–78.5 | 112,980,785–115,691,953 | 2.95 | 4.6 | 2.84 | KUI3 | |
| q2PH8-1 | 8 | LS | ET | 45.8 | PZE-108015015- PZE-108018250 | 43.9–50 | 14,753,943–17,383,010 | 6.67 | 12.15 | -4.52 | B77 | |
| WQ | ET | 5.36 | 11.47 | -3.37 | ||||||||
| q2PH8-2 | 8 | WQ | ET | 63 | PZE-108046118- PZE-108054764 | 59.9–63.4 | 76,039,534–97,777,183 | 5 | 8.98 | -4.24 | B77 | |
| q2PH8-3 | 8 | LS | CK | 71.7 | PZE-108064061- SYNGENTA14910 | 70.8–72 | 114,297,738–118,401,344 | 7.22 | 14.47 | -5.2 | B77 | |
| WQ | CK | 6.35 | 11.51 | -6.32 | ||||||||
| q2PH9-1 | 9 | LS | ET | 48.3 | PZE-109022525- PZE-109024455 | 46.3–49.6 | 23,008,509–24,487,068 | 6.12 | 10.77 | 4.33 | KUI3 | |
| WQ | ET | 4.21 | 7.06 | 3.84 | ||||||||
| LS | CK | 6.38 | 12.14 | 4.2 | ||||||||
| WQ | CK | 5.41 | 10.12 | 5.32 | ||||||||
| q2PH9-2 | 9 | LS | CK | 62.9 | PZB02480.2- PZE-109071675 | 62.1–64.5 | 107,416,972–116,353,876 | 3.97 | 7.56 | 3.77 | KUI3 | |
| EH | q2EH2-1 | 2 | LS | ET | 47.4 | SYN29778- SYN29038 | 46.5–51.7 | 17,793,282–20,724,795 | 4.76 | 8.69 | -2.47 | B77 |
| q2EH2-2 | 2 | WQ | CK | 66.9 | PZE-102054526- SYN34233 | 66.1–67.5 | 32,516,815–36,590,474 | 5.08 | 9.12 | -3.22 | B77 | |
| LS | CK | 3.04 | 5.81 | -2.47 | ||||||||
| q2EH2-3 | 2 | WQ | CK | 129.2 | PZE-102134800- PZE-102139383 | 126.3–130.1 | 184,968,231–187,633,354 | 3.08 | 5.75 | 1.51 | KUI3 | |
| WQ | ET | 2.89 | 5.07 | 1.72 | ||||||||
| LS | CK | 3.56 | 6.24 | 2.97 | ||||||||
| LS | ET | 3.25 | 5.33 | 2.86 | ||||||||
| q2EH3 | 3 | WQ | ET | 1.8 | PZE-103000307- PZE-103001968 | 1.5–3.9 | 1,233,964–1,978,736 | 2.52 | 4.55 | -1.35 | B77 | |
| q2EH5 | 5 | WQ | CK | 164.4 | PZE-105156970- PZE-105158756 | 163.7–166.8 | 205,776,348–206,557,530 | 5.45 | 9.98 | 2.93 | KUI3 | |
| LS | CK | 3.69 | 7.18 | 2.8 | ||||||||
| q2EH6 | 6 | LS | CK | 116.1 | SYN23640- PZE-106112056 | 113.6–117.8 | 156,879,515–159,464,206 | 3.95 | 7.6 | -2.8 | B77 | |
| WQ | CK | 4.81 | 8.56 | -2.77 | ||||||||
| WQ | ET | 9.18 | 17.71 | -2.67 | ||||||||
| LS | ET | 2.74 | 4.47 | -1.72 | ||||||||
| q2EH7 | 7 | LS | ET | 96.1 | PZE-107074506- SYN32297 | 94.6–99.1 | 130,360,641–133,374,241 | 5.64 | 10.16 | 2.63 | KUI3 | |
| WQ | ET | 2.64 | 4.46 | 1.37 | ||||||||
| LS | CK | 3.11 | 5.93 | 2.52 | ||||||||
| WQ | CK | 5.32 | 9.54 | 2.41 | ||||||||
| q2EH9 | 9 | LS | CK | 62.5 | PZE-109065808- PZE-109066235 | 62.5–62.9 | 108,522,923–109,304,376 | 3.28 | 6.28 | 2.6 | KUI3 | |
| WQ | ET | 3.51 | 6.41 | 1.62 | ||||||||
| LS | ET | 5.1 | 9.18 | 2.55 | ||||||||
| WQ | CK | 4.32 | 7.54 | 2.01 | ||||||||
| ILAU | q2ILAU1-1 | 1 | LS | ET | 19 | SYN13185- SYN13395 | 19.0–20.3 | 6,844,116–7,565,560 | 2.82 | 5.49 | -2.56 | B77 |
| q2ILAU1-2 | 1 | LS | CK | 74 | PZE-101046080- SYN23289 | 71.3–75.6 | 31,850,441–38,871,055 | 5.06 | 10.28 | -3.48 | B77 | |
| q2ILAU2-1 | 2 | WQ | ET | 49.8 | PZE-102040312- PZE-102043154 | 48.9–52 | 20,103,068–21,874,416 | 4.08 | 7.54 | -2.9 | B77 | |
| q2ILAU2-2 | 2 | LS | CK | 180.9 | SYN28307- PZE-102186367 | 176.9–181.5 | 225,537,147–230,291,137 | 4.09 | 7.92 | -3.01 | B77 | |
| LS | ET | 4.5 | 9.06 | -3.42 | ||||||||
| WQ | CK | 4.09 | 7.92 | -3.01 | ||||||||
| WQ | ET | 4.5 | 9.06 | -3.42 | ||||||||
| q2ILAU6 | 6 | LS | ET | 141.7 | PHM5361.13- PZE-106129549 | 139.8–141.7 | 166,592,099–167,325,854 | 2.86 | 5.63 | 2.61 | B77 | |
| q2ILAU8-1 | 8 | WQ | ET | 63 | PZE-108049474- PZE-108055459 | 61.1–64.2 | 85,149,829–99,749,632 | 6.05 | 11.34 | -3.42 | B77 | |
| q2ILAU8-2 | 8 | WQ | CK | 69.5 | SYN39345- PZE-108066080 | 67.1–71.7 | 106,271,779–117,871,384 | 4.65 | 9.74 | -3.5 | B77 | |
| LS | CK | 6.82 | 13.8 | -3.99 | ||||||||
| q2ILAU9 | 9 | WQ | ET | 47.3 | PZE-1090212740 SYN11956 | 45.4–50.7 | 21,678,811–25,817,148 | 4.8 | 10.87 | 2.9 | KUI3 | |
| LS | ET | 4.77 | 8.67 | 3.22 | ||||||||
| WQ | CK | 4.72 | 9.94 | 3.98 | ||||||||
| LS | CK | 6.94 | 13.02 | 4.27 | ||||||||
| K22 × BY815 | ||||||||||||
| ILAU | q1ILAU2-1 | 2 | WQ | CK | 41.5 | PZE-102027559- PZE-102028305 | 41.5–41.8 | 12,801,930–13,300,441 | 4.48 | 7.46 | 2.7 | BY815 |
| LS | CK | 2.98 | 4.92 | 2.29 | ||||||||
| q1ILAU2-2 | 2 | WQ | CK | 180.5 | PZE-102192366- PZE-102193904 | 179.2–182.7 | 234,718,008–236,615,130 | 4.61 | 10.82 | -2.73 | K22 | |
| LS | ET | 5.37 | 8.71 | -4.05 | ||||||||
| LS | CK | 4.63 | 8.12 | -2.95 | ||||||||
| WQ | ET | 6.07 | 11.07 | -3.03 | ||||||||
| q1ILAU3 | 3 | WQ | CK | 70.5 | SYN12568- PZE-103027704 | 61–71.1 | 12,189,577–20,378,743 | 3.13 | 4.76 | -2.1 | K22 | |
| q1ILAU6 | 6 | LS | CK | 21.9 | PZE-106020696- PZE-106013766 | 18.3–28.8 | 16,795,395–34,863,238 | 4.58 | 7.7 | 3.75 | BY815 | |
| LS | ET | 4.86 | 7.65 | 3.73 | ||||||||
| WQ | CK | 4 | 6.58 | 2.48 | ||||||||
| WQ | ET | 3.92 | 6.74 | 2.35 | ||||||||
| q1ILAU7-1 | 7 | LS | ET | 54.3 | PZE-107015084- SYN27395 | 49.7–55.2 | 11,799,137–17,697,488 | 3.53 | 6.19 | 3.34 | BY815 | |
| LS | CK | 3.85 | 6.65 | 3.73 | ||||||||
| WQ | ET | 3.68 | 5.81 | 2.48 | ||||||||
| WQ | CK | 3.23 | 5.7 | 2.35 | ||||||||
| q1ILAU7-2 | 7 | WQ | CK | 70.1 | PZE-107068961- SYN28758 | 68–70.8 | 125,861,581–129,967,283 | 3.32 | 5.48 | 2.29 | BY815 | |
| LS | CK | 4.06 | 6.9 | 2.75 | ||||||||
| q1ILAU8 | 8 | WQ | CK | 94.8 | PZE-108099425- PZE-108105216 | 90.3–98.3 | 155,643,006–159,952,552 | 3.7 | 6.12 | -2.47 | K22 | |
| LS | CK | 4.35 | 7.35 | -2.89 | ||||||||
| WQ | ET | 2.77 | 4.73 | -2.1 | ||||||||
| LS | ET | 5.03 | 8.43 | -3.62 | ||||||||
| q1ILAU9-1 | 9 | LS | CK | 50.9 | PZE-109027610- PZE-109055561 | 46.8–52.4 | 28,005,182–89,325,234 | 3.6 | 6.41 | 2.55 | BY815 | |
| WQ | ET | 3.59 | 6.26 | 2.29 | ||||||||
| LS | ET | 5.57 | 9.04 | 4.03 | ||||||||
| WQ | CK | 3.81 | 7.22 | 2.72 | ||||||||
| q1ILAU9-3 | 9 | WQ | CK | 66.3 | PZE-109077680- SYN12671 | 66.3–69.4 | 125,169,744–128,964,637 | 2.53 | 3.87 | 1.91 | BY815 | |
| q1ILAU10 | 10 | WQ | CK | 113.4 | PZE-110103320- PZE-110103696 | 113.4–113.9 | 146,173,534–146,292,761 | 2.67 | 4.08 | -1.96 | K22 | |
PH plant height, EH ear height, ILAU the internode length above the uppermost ear.
a Location QTL detected in Wuqiao (WQ) and Lishu (LS).
b Treatment CK, without ethylene (CK) treatments; ET, with ethylene (ET) treatments.
c Position The peak position with the highest LOD of each QTL.
d Marker Interval Flanking markers, the left and right markers of the QTL.
e Support interval Genetic position interval of each QTL.
f Physical interval Physical location of the QTL in the maize genome (MaizeDB; http://www.maizegdb.org/).
g LOD scores logarithm of the odds (to the base 10).
h R2 (%) rate of contribution were calculated for each QTL.
i A additive effect, positive values indicated that BY815 or KUI3 carries the allele for an increase in the traits, while negative values(-) indicated that K22 or B77 contributed the allele for an increase in the trait value.
j Positive allele effect carries of parental.