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. 2018 Feb 21;13(2):e0193156. doi: 10.1371/journal.pone.0193156

Fig 9. Results of TUNEL test in root cells of barley seedlings cv. ‘Sebastian’.

Fig 9

DAPI stained nuclei (A-H), with or without green fluorescence as a result of TUNEL reaction (A`-H`). (A, A`) control nuclei showing weak green fluorescence, (B, B`) positive control (DNAse solution was used to induce DNA strand breaks), (C, C`) negative control (nucleotide solution, without terminal transferase was used), (D, D`) not-treated cells, (E, E`) after treatment with 30 μM Al (nuclei with and without green fluorescence are shown), (F, F`) after treatment with 40 μM Al (nuclei with and without green fluorescence are shown) (G, G`) fragmented nucleus after treatment with 30 μM Al with green fluorescence, (H, H`) nuclei with micronucleus, both show green fluorescence. Bars represent 20 μm.