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. 2018 Feb 21;13(2):e0191761. doi: 10.1371/journal.pone.0191761

Fig 7. Detection of pyrrolnitrin in extracellular culture extract of the antagonistic S. marcescens strain ETR17 by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) analysis.

Fig 7

The combined column fractions F10 and F11 obtained previously from silica gel column chromatography of the crude culture extract was analysed using a gradient elution program with methanol and ammonium acetate buffer at a flow rate of 0.8 mL min-1 at 254 nm to obtain a major peak at retention time 14.85 minutes corresponding to pyrrolnitrin. Mass spectra showing molecular ion peak at m/z 259 (M + 2H)+ indicating pyrrolnitrin.