TABLE 1.

Lineage tracing methods in the study of hematopoiesis.

Approach	Utility in hematopoietic development
Mass Cytometry (CyTOF)	Allows for simultaneous identification of multiple cell types from heterogeneous sample of blood
Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF)	Analyzes protein contents in samples and elucidates glycosylation patterns
Single-cell RNA Sequencing	Investigates cell-to-cell variation at transcription level
CRISPR/Cas9-based Genome Editing	Used to knock out hematopoietic genes and the β-globin gene in human HSCs and allows for multiple genetic modifications
Polylox Barcoding	To study origin and clonal composition of HSCs
Multiplexed Fluorescent Labeling and Sequencing	The single-cell transplant data are coupled with single-cell gene expression analysis on different cells to resolve subpopulations with corresponding gene expression and repopulation potential
Tissue Engineering & 3D Scaffold Development	Recreates 3D microenvironment conducive for HSC formation, expansion, development, and differentiation.