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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: J Immunol. 2018 Jan 19;200(5):1929–1936. doi: 10.4049/jimmunol.1700812

Figure 2.

Figure 2

KEAP1 editing resulted in significant NRF2 activation in primary T cells. We used target site 2 (T2) specific RNP complex containing ATTO 550 labeled tracrRNA in primary T cells (n=3). ATTO 550 labeled tracrRNA forms an integral part of the RNP complex and provided better assessment of transfection efficiency. (A) Microscopic and flow cytometric analysis of primary T cell 24h after electroporation showed upto ~80% cells received RNP complex. (B) KEAP1 editing analysis using Surveyor mutation assay showed cleavage in about 40% cells that received RNP complex in comparison to control cells. (C) There was a significant increase in NQO1 (p≤0.01; 16 fold), HO1 (p≤0.01; 9 fold) and GCLM (p≤0.04; 2 fold) mRNA expression in primary T cell, 72h after RNP complex electroporation, compared to control cells.