Table 1.
Oligonucleotide primer and E. coli strain list.
| Name | Oligo Sequence |
|---|---|
| Sugar1 | CGCTCTAGAAGATTATCTCCAATAGCGCTTGGT |
| Sugar2 | GATCACAGATTATCTCCAATAGCGCTTGG |
| cat-MfeI1 | GCGCAATTGGTGTAGGCTGGAGCTGCTTC |
| cat-MfeI2 | GCGCAATTGCATATGAATATCCTCCTTAG |
| vioB Homo1 | ATGGCCTATTTAGATGAAATACAGTT |
| vioB Homo2 | TCACAGATTATCTCCAATAGCGCTTGG |
| wecD&E-NcoI | GCGCCATGGGTGCAGGCCAAAATTGCGGCATCA |
| wecD&E-XhoI | CGCCTCGAGTCAGGAAAAGTAGTTCAACAAAGTCGC |
| kan-BsaI | CGCCACAAGAGACCTGTGTAGGCTGGAGCTGCTTCG |
| kan-EcoRV | CGCGATATCCATTATGAATATCCTCCTTA |
| wecD&E Homo1 | GTGCAGGCCAAAATTGCGGCATCA |
| wecD&E Homo2 | TCAGGAAAAGTAGTTCAACAAAGTCGC |
| rmlA/D-BamHI | CGCGGATCCATGAATATCCTCCTATTCGGCAAAAC |
| rmlA/D-NotI | CGCGCGGCCGCATTAATAACCTTTAATCATTTTGAGCAGATAC |
| Kan-rmlD1 | GCGGTCGACTGTGTAGGCTGGAGCTGCTTCG |
| Kan-rmlD2 | GCGTACGTACATTATGAATATCCTCCTTAG |
| rmlA/D1 | ATGAATATCCTCCTATTCGGCAAAAC |
| rmlA/D2 | ATTAATAACCTTTAATCATTTTGAGCAG |
| kan-spec1 | CCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGTGTAGGCTGGAGCTGCTTCG |
| kan-spec2 | TTATTTGCCGACTACCTTGGTGATCTCGCCTTTCACGTAGTGGACAAATTCCCATTATGAATATCCTCCTTAG |