Figure 2. Allergen-primed lung CD4+TRM localize around airways.
(A) Upper: H&E stained lung slices from PBS sensitized, HDM sensitized and HDM memory mice (upper panels). Lower: Confocal microscope images of lung sections stained with Fluorescein labeled ECL (green) and anti-CD4 (white) and their association with the lung airway (AW) and blood vessel (B). Images are representative of 8 mice from 5 independent experiments. Scale bar represents 100μm. (B) Number of CD4 T cells (mean ± SEM) surrounding broncho-vascular bundles for each group (N= 6–11 bronchovascular bundles per group compiled from 5 independent experiments)(* p<0.1. **** p<0.0001, 1-way ANOVA).