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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: J Immunol. 2018 Jan 17;200(5):1561–1569. doi: 10.4049/jimmunol.1700257

Figure 3. Increased airway hyper-responsiveness and rapid influx of granulocytes and lymphocytes into the airways following re-exposure to allergen.

Figure 3

(A) Airway hyper-responsiveness following methacholine challenge of mouse groups previously exposed to PBS or HDM for 3 weeks (“HDM memory”), then rested and challenged with PBS or HDM after 2 days (2d) (n= 4–11 mice per group compiled from 3 independent experiments) (* p<0.01, ** p<0.01, 2-way ANOVA). (B) Frequency of lymphocytes (low FSC, low SSC) and granulocytes (low FSC, high SSC) in the BAL of HDM memory mice following 2d HDM challenge (upper). Representative flow cytometry analysis (lower) of CD4+ and CD8+T cells in the BAL, representative of 4 independent experiments (C) Cellular content of the BAL in naïve and HDM memory mice following 2d HDM challenge as in (A). Graph shows the numbers (mean±SEM) of indicated cell types within the BAL of naïve and HDM memory mice following 2d PBS or HDM challenge (n= 4–8 mice per group compiled from 2 independent experiments).