Fig. 1.

a Effect of different electroporation parameters on transformation efficiency of A. tumefaciens MOG301 electrocompetent cells. The mean differences are statistically significant at 0.05 level, denoted by different letters. Statistical analysis was performed using one-way ANOVA and LSD posthoc test. The results show the average of three experiments and three replicates for each. b Effect of incubation time of bacterial suspension on the number of transformants. After electroporation, bacterial suspension was diluted with 1 mL SOC media and incubated for 60–180 min. The number of transformants were calculated after dilution and plating. Data represent the mean of two independent biological experiments with three replicates for each. The mean difference is statistically significant at 0.05 level. Statistical analysis was performed using one-way ANOVA and Tukey’s posthoc test. Asterisk indicates statistically significant differences between 60, 120, and 180 min incubation times