Fig. 6.

Characterization of the pluripotency of selected iPSC lines. a Principal component analysis of the RNA-Seq gene expression data performed on selected iPSC lines (orange circles) and their corresponding parental fibroblasts (blue squares), as well as on two human ESC lines (H1 and H9, black triangles), documenting the close clustering of iPSCs with H1 and H9. All IN2 iPSC lines were derived from FN2, I50 from F50, and I50S from F50S (see also Supplementary Table 1). b Immunofluorescent analysis showing expression of selected pluripotency markers in the indicated iPSC lines. H9 human ESCs and FN2 fibroblasts are included as positive and negative controls, respectively. See Fig. 5c and Supplementary Figs. 11 and 12 for the analysis of additional iPSC lines. c Bisulfite sequencing of the OCT4 promoter region in a selected iPSC line and its parental fibroblast line. H9 human ESCs are included as a control. The closed circles indicate methylated sites of the analyzed region. d Immunostaining showing expression of the neuronal marker βIII-Tubulin (TUJ1) (ectoderm), and the endoderm-specific cytokeratin Endo-A in the indicated iPSC lines subjected to directed differentiation. e Hematoxylin and eosin staining and immunofluorescent analysis of consecutive sections of teratomas derived from indicated iPSC lines showing histology and marker expression specific to ectoderm (βIII-Tubulin (TUJ1), neural tissues), mesoderm (vimentin, connective tissues), and endoderm (Endo-A, endothelium). See Fig. 5e and Supplementary Fig. 13 for teratoma analysis of additional iPSC lines. All scale bars, 250 µm