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. 2018 Feb 21;8:3398. doi: 10.1038/s41598-018-21768-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NDRG1 expression decreased HER2 level in both SK-OV-3 and SK-BR-3 cells. (A) Western blot indicating the expression of NDRG1 in SK-OV-3 and SK-BR-3 cells. (B) Quantification of western blot signal intensity analysis is expressed relative to the β-actin loading control by using Image J software. (C) Suppression of NDRG1 by siRNA leaded to increase in the level of HER2 in SK-OV-3 cells. SK-OV-3 cells were transiently transfected with nonspecific control siRNA (Ctrl) or NDRG1 siRNA (siNDRG1) for 72 h at 37 °C. (D) NDRG1 expression inhibits HER2 expression in SK-BR-3 cells. The plasmid of pCDNA3.1-NDRG1 or the vector control (Ctrl) was constructed and transfected into SK-BR-3 cells. (E) Quantification of western blot signal intensity analysis in SK-OV-3 cells is expressed relative to the β-actin loading control by using Image J software. (F) Quantification of western blot signal intensity analysis in SK-BR-3 cells is expressed relative to the β-actin loading control by using Image J software. Data show the mean ± SD (3 independent experiments); *p < 0.05; **p < 0.01; ***p < 0.001.