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. 2018 Feb 21;8:3398. doi: 10.1038/s41598-018-21768-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of DpdtC on suppressing HER2 expression is dependent on NDRG1 in SK-OV-3 cells. (A) SK-OV-3 cells were transfected with control siRNA or siNDRG1 for 72 h at 37 °C, followed by treatment with DpdtC (2 μM) for 30 h at 37 °C. Then levels of NDRG1 and HER2 were tested by western blot. (B) Quantification of western blot signal intensity analysis is expressed relative to the β-actin loading control by using Image J software. Data show the mean ± SD (3 independent experiments); **p < 0.01; ***p < 0.001.