. 2018 Feb 21;8:3406. doi: 10.1038/s41598-018-21783-2

Table 1.

Primer pairs used in the RT-qPCR.

Genes	Primer sequences (5′ to 3′)
Human
Sox 9	Forward: CTCTGGAGACTTCTGAACGAGAGC
Sox 9	Reverse: GTTCTTCACCGACTTCCTCCG
Collagen type II	Forward: TGAAGGTGCTCAAGGTCCTC
Collagen type II	Reverse: ATTCCATCTGTTCCAGGGTT
Aggrecan	Forward: GCCAGCACCACCAATGTAAG
Aggrecan	Reverse: TTCAGTAACACCCTCCACGA
GAPDH	Forward: GGTGACTAACCCTGCGCTC
GAPDH	Reverse: CAAATGAGCCCCAGCCTTCTC
Mouse
IL-10	Forward: GAGAAGCATGGCCCAGAAATC
IL-10	Reverse: GAGAAATCGATGACAGCGCC
IL-1ra-	Forward: CTCCAGCTGGAGGAAGTTAAC
IL-1ra-	Reverse: CTGACTCAAAGCTGGTGGTG
TNF-α	Forward: CTGAACTTCGGGGTGATCGG
TNF-α	Reverse: GGCTTGTCACTCGAATTTTGAGA
IL-1β	Forward: TGGAGAGTGTGGATCCCAAG
IL-1β	Reverse: GGTGCTGATGTACCAGTTGG
IL-6	Forward: ATAGTCCTTCCTACCCCAATTTCC
IL-6	Reverse: GATGAATTGGATGGTCTTGGTCC
β-actin	Forward: AGTGTGACGTTGACATCCGTA
β-actin	Reverse: GCCAGAGCAGTAATCTCCTTCT