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. 2018 Feb 21;8:3411. doi: 10.1038/s41598-018-21648-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cross-sectional histological staining and confocal laser scanning microscopy of the infected clot model. Both a and b are each composed of two combined microscope images acquired at 40x magnification. (a) Image of H&E staining of an infected clot cross-section. The arrowhead indicates bacteria (purple), the arrow points at fibrin (pink), and a dashed-line arrow at erythrocytes (red). (b) Crystal violet staining, where an arrowhead indicates bacteria (purple), an arrow for fibrin (yellow), and a dashed-line arrow for erythrocytes (brown). (c) ImageJ maximum intensity projection of live (green, SYTO 9 stained) and dead (red, PI stained) S. aureus comprising the outer layer of a representative infected clot.