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. 2018 Feb 21;8:3411. doi: 10.1038/s41598-018-21648-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bright-field light microscopy imaging of infected clots acquired directly following treatment. Infected clot treated only with plasma at 4x (a) and at 10x magnification (b). The black arrow in image b points out the biofilm (beige). (c) A plasma, rt-PA, and oxacillin treated infected clot at 4x magnification. (d) A plasma, rt-PA, oxacillin, ultrasound, and Definity treated infected clot at 4x magnification. The sutures (black line) are situated at the bottom of the clots, which can be observed to the right of the clot in images a–c.