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. 2018 Feb 21;8:3418. doi: 10.1038/s41598-018-21757-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Custom algorithms were created to identify histological features of pathological significance. (A) CD45^+ve inflammatory cells have higher optical density of DAB compared to CD45^−ve cells. (B) Areas with collagen deposition have higher optical density of Sirius Red compared to collagen free areas. (C,D) Necrotic areas and fatty areas have lower optical density of hematoxylin compared to parenchyma lacking these features. For (B–D), ten FOVs from each sample was used for analysing fibrotic, fatty and necrotic areas, and compared to ten FOVs of parenchyma lacking these features. ***p < 0.0001.